Bioconductor Cell lines

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plotMDS Cell lines limma

updated 6.4 years ago by Gordon Smyth 52k • written 6.4 years ago by Alexander Azarian ▴ 30

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Comment: How can I generate a heatmap and clustering of differentially expressed genes in by Renuka • 0

Thanks Kelvin i had same doubt .Do i need to do log2 (FPKMcounts) for this

Comment: Is there a way to do DEG analysis across two batches sharing one sample only? by georgeyu703 • 0

Michael, Thank you very much!

Comment: Is there a way to do DEG analysis across two batches sharing one sample only? by Michael Love 43k

Yes, with the knowledge that, it is expected to be underpowered.

Comment: Is there a way to do DEG analysis across two batches sharing one sample only? by georgeyu703 • 0

Thank you, Michael. How? Could you provide more details? Can I just do it as usual, I mean to include batch in the model?

Answer: Is there a way to do DEG analysis across two batches sharing one sample only? by Michael Love 43k

The short answer is yes, you can use even a single sample to estimate the batch effect (and therefore control for it in otherwise confounde…

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Answer: EdgeR Biological CVs

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